NEFH ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for NEFH ELISA 试剂盒 (ABIN8070227)
抗原
See all NEFH ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 0.2 pg/mL
-
原理
- Phospho-NFH Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human phosphorylated neurofilament heavy polypeptide (NFH) in lysate samples.
-
Analytical Method
- Quantitative
-
特异性
- This ELISA antibody pair detects human Phospho NFH.
-
组件
-
- Anti-Pan-NFH Microplate
- Phospho-NFH Standard
- Anti-Phospho NFH Detection Antibody
- HRP-Streptavidin
- Wash Buffer
- Assay Diluent B
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Anti-Pan-NFH Microplate
- Phospho-NFH Standard
- Anti-Phospho NFH Detection Antibody
- HRP-Streptavidin
- Wash Buffer
- Assay Diluent B
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
-
试剂未包括
-
- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare the positive control or sample dilutions
- Distilled or deionized water
- Shaker
-
-
Neurofilament, Heavy Polypeptide (NEFH) ELISA Kit
NEFH 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cerebrospinal Fluid, Plasma, Serum
Neurofilament, Heavy Polypeptide (NEFH) ELISA KitNEFH 适用: 人 phosphorylated Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Neurofilament, Heavy Polypeptide (NEFH) ELISA KitNEFH 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Neurofilament, Heavy Polypeptide (NEFH) ELISA KitNEFH 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Neurofilament, Heavy Polypeptide (NEFH) ELISA KitNEFH 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Neurofilament, Heavy Polypeptide (NEFH) ELISA KitNEFH 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Neurofilament, Heavy Polypeptide (NEFH) ELISA KitNEFH 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Neurofilament, Heavy Polypeptide (NEFH) ELISA KitNEFH 适用: 犬 Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Neurofilament, Heavy Polypeptide (NEFH) ELISA KitNEFH 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Neurofilament, Heavy Polypeptide (NEFH) ELISA KitNEFH 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and positive control as instructed.
- Add 100 μL of each standard and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
-
-
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
-
别名
- NFH
-
背景
-
Gene Symbols: NEFH, KIAA0845, NFH
Background: Phospho-NFH Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human phosphorylated neurofilament heavy polypeptide (NFH). An anti-pan NFH antibody has been coated onto a 96-well plate. Standard and Samples are pipetted into the wells and NFH present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phospho-NFH (KSP site) antibody is used to detect phosphorylated NFH. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NFH bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Neurofilament heavy polypeptide (NF-H) (200 kDa neurofilament protein) (Neurofilament triplet H protein)
-
基因ID
- 4744
-
UniProt
- P12036
抗原 See all NEFH ELISA试剂盒
-
