MSH alpha ELISA 试剂盒
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Quick Overview for MSH alpha ELISA 试剂盒 (ABIN8070082)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.98 pg/mL
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原理
- Alpha-MSH Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting human, mouse, or rat melanocyte-stimulating hormone alpha based on the competitive ELISA principle. The immunoassy is optimized for serum, plasma and cell culture media.
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Analytical Method
- Quantitative
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特异性
- This EIA kit is designed to detect human mouse, and rat Alpha-Melanocytestimulating hormone.
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灵敏度
- 20.5 pg/mL
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450 nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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MSH alpha ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Plasma, Serum
MSH alpha ELISA Kit适用: 人 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 人 Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 兔 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 人 Colorimetric Competition ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Suggested Plasma Dilution: Human: 4X / Mouse: 8X / Rat: 8X
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Standard, Biotinylated Alpha-MSH peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2°C to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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- MSH alpha
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别名
- alpha-MSH
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背景
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Gene Symbols: STAMBP, AMSH
Background: Alpha-Melanocyte-stimulating hormone (alpha-MSH), a proteolyic cleavage product from ACTH (1-13), is an endogenous peptide hormone and neuropeptide of the melanocortin family. It is a non-selective full agonist of the melanocortin receptors MC1, MC3, MC4, and MC5, but not MC2. Alpha-MSH is important for pigmentation primarily of the hair and skin. It also plays a role in feeding behavior, energy homeostasis, sexual activity, and protection against ischemia and reperfusion injury.
In this assay, a biotinylated Alpha-MSH peptide is spiked into the samples and standards. The samples and standards are then added to the plate, where the biotinylated Alpha-MSH peptide competes with endogenous (unlabeled) Alpha-MSH for binding to the anti-Alpha-MSH antibody. After a wash step, any bound biotinylated Alpha-MSH then interacts with horseradish peroxidase (HRP)- streptavidin, which catalyzes a color development reaction. The intensity of the colorimetric signal is directly proportional to the amount of captured biotinylated Alpha-MSH peptide and inversely proportional to the amount of endogenous Alpha- MSH in the standard or samples. A standard curve of known concentration of Alpha-MSH peptide can be established and the concentration of Alpha-MSH peptide in the samples can be calculated accordingly.
Protein Name (Synonyms): alpha-melanocyte stimulating hormone (a-MSH/ aMSH)
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基因ID
- 10617
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UniProt
- O95630
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途径
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
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