IFNE ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for IFNE ELISA 试剂盒 (ABIN8069826)
抗原
See all IFNE ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 0.01 ng/mL
-
原理
- The Human IFN-epsilon ELISA Kit is a pre-coated sandwich-based assay kit designed to quantitatively measure IFN-epsilon (IFNE) levels in human cell culture media, plasma, and serum.
-
Analytical Method
- Quantitative
-
特异性
- This ELISA antibody pair detects human IFN-epsilon. Other species not determined.
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
- Adhesive Sealing Film
-
试剂未包括
-
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard or sample dilutions
- Plastic wrap
-
-
Interferon epsilon (IFNE) ELISA Kit
IFNE 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interferon epsilon (IFNE) ELISA KitIFNE 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Suggested Plasma Dilution: 2 fold
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
-
-
- IFNE (Interferon epsilon (IFNE))
-
别名
- IFN-epsilon
-
背景
-
Gene Symbols: IFNE
Protein Name (Synonyms): Interferon epsilon (IFN-epsilon) (Interferon epsilon-1)
-
UniProt
- Q86WN2
-
途径
- Hormone Activity
抗原 See all IFNE ELISA试剂盒
-
