IgG ELISA 试剂盒
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北京 101111
Quick Overview for IgG ELISA 试剂盒 (ABIN8069312)
抗原
See all IgG ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- COVID-19 Spike Variant Human IgG ELISA Kit 13 is a human antibody detection kit (indirect ELISA method) for the quantitative measurement of human IgG against the following spike protein mutations of SARS-CoV-2: N440D. This Product is for Research Use Only. Not for use in in vitro diagnostics.
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Analytical Method
- Semi-Quantitative
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组件
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- SARS-CoV-2 Protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Anti-Human IgG
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
- SARS-CoV-2 Protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Anti-Human IgG
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Distilled or deionized water
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IgG ELISA Kit
适用: 兔 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Rabbit TrueBlot® Set (with IP beads)WB, IP 适用: 兔
Mouse TrueBlot® Set (with IP beads)WB, IP 适用: 小鼠
IgG ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: Cow Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: 人 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鸡 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
- Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -80 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
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- IgG
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物质类
- Antibody
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背景
- Gene Symbols: IGG
抗原 See all IgG ELISA试剂盒
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