HCP ELISA 试剂盒
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Quick Overview for HCP ELISA 试剂盒 (ABIN8069083)
抗原
See all HCP products适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.25 ng/mL
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原理
- CHO Host Cell Proteins (HCP) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of process-related HCP impurities derived from production processes based on CHO (Chinese Hamster Ovaries) production cell lines.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects Chinese Hamster Ovaries (CHO) HCP. The antibody has been determined by Western Blot of analysis, 2-D Gel separation.
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Host Cell Proteins (HCP) ELISA Kit
HCP 适用: 大肠杆菌（E. Coli） Colorimetric Sandwich ELISA 2.6 ng/mL – 250.0 ng/mL
Host Cell Proteins (HCP) ELISA KitHCP 适用: CHO cells Colorimetric Sandwich ELISA 3.125 ng/mL - 400 ng/mL Biological Therapeutics, Cell Culture Supernatant, Purified Protein
Host Cell Proteins (HCP) ELISA KitHCP 适用: E. coli Colorimetric Sandwich ELISA 1.95 ng/mL - 125 ng/mL Biological Therapeutics, Cell Culture Supernatant, Purified Protein
Host Cell Proteins (HCP) ELISA KitHCP 适用: Sf9 insect cell line Colorimetric Sandwich ELISA Biological Therapeutics, Cell Culture Supernatant, Purified Protein
Host Cell Proteins (HCP) ELISA KitHCP 适用: Pichia pastoris Colorimetric Sandwich ELISA Biological Therapeutics, Cell Culture Supernatant, Purified Protein
Host Cell Proteins (HCP) ELISA KitHCP 适用: HEK 293 Cells Colorimetric Sandwich ELISA Biological Therapeutics, Cell Culture Supernatant, Purified Protein
Host Cell Proteins (HCP) ELISA KitHCP 适用: Saccharomyces cerevisiae Colorimetric Sandwich ELISA 6.25 ng/mL - 800 ng/mL Biological Therapeutics, Cell Culture Supernatant, Purified Protein
Host Cell Proteins (HCP) ELISA KitHCP 适用: Ogataea polymorpha Colorimetric Sandwich ELISA 6.25 ng/mL - 800 ng/mL Biological Therapeutics, Cell Culture Supernatant, Purified Protein
Host Cell Proteins (HCP) ELISA KitHCP 适用: Pichia pastoris Colorimetric Sandwich ELISA 6.25 ng/mL - 800 ng/mL Biological Therapeutics, Cell Culture Supernatant, Purified Protein
Host Cell Proteins (HCP) ELISA KitHCP 适用: CHO cells Colorimetric Sandwich ELISA 0.78 ng/mL - 200 ng/mL Biological Therapeutics, Cell Culture Supernatant, Purified Protein
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- HCP (Host Cell Proteins (HCP))
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别名
- Host Cell Proteins
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背景
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Background: This assay employs an antibody specific for CHO HCP coated on a 96- well plate. Standards and samples are pipetted into the wells and CHO HCP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-CHO HCP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CHO HCP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Host Cell Proteins (HCP)
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