GLP-1 ELISA 试剂盒
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北京 101111
Quick Overview for GLP-1 ELISA 试剂盒 (ABIN8068912)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.1 pg/mL
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原理
- Human/Mouse/Rat total Glucagon-like peptide-1 EIA Kit for optimized for serum and cell culture medium. Competition-based ELISA
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Analytical Method
- Quantitative
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特异性
- This kit measures total GLP-1, including the full-length (1-37), active (7-36), and inactive (9-36) forms. The antibody shows similarly high affinity for each form. No cross-reactivity observed with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
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灵敏度
- 0.288 pg/mL
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Lyophilized Standard
- Capture Antibody
- Assay Diluent
- Biotinylated Peptide
- HRP-Streptavidin
- Positive Control
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1-liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Software which can perform four-parameter logistic regression models
- Tubes to prepare standard or sample dilutions
- Orbital shaker
- Aluminum foil
- Plastic wrap
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Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitSmall Sample GLP-1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 AA 7-36 Colorimetric Sandwich ELISA 0.06-54 pM/L Plasma
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应用备注
- Suggested Plasma Dilution: Human: 2X / Mouse: 2X / Rat: 2X
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL Capture Antibody to each well. Incubate 1.5 hours at room temperature or overnight at 4 °C.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C.
- Add 100 μL prepared Streptavidin solution. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.
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- GLP-1 (Glucagon-like peptide 1 (GLP-1))
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别名
- GLP-1
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背景
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Gene Symbols: GCG
Background: Glucagon-like peptide-1 (GLP-1) is a highly conserved amino-acid-long peptide hormone among mammals and mainly secreted from the intestinal L-cells and the CNS. Functionally, GLP-1 plays an important role in energy homeostasis and nutrient absorption. The initial product GLP-1 (1-37) is susceptible to amidation and proteolytic cleavage, which gives rise to the two truncated and equipotent biologically active forms, GLP-1 (7-36) amide and GLP-1 (7-37). Then, the active forms of GLP-1 are extremely susceptible to the catalytic activity of the proteolytic enzyme dipeptidyl peptidase-4 (DPP-4), resulting in the abundant inactivated GLP-1 (9-36) amide constituting 60-80 % of total GLP-1 in circulation. This kit measures total GLP-1 including full length GLP-1 (1-37), the active (7-36) and inactive (9-36) forms of GLP-1.
Protein Name (Synonyms): Pro-glucagon [Cleaved into: Glicentin, Glicentin-related polypeptide (GRPP), Oxyntomodulin (OXM) (OXY), Glucagon, Glucagon-like peptide 1 (GLP-1) (Incretin hormone), Glucagon-like peptide 1(7-37) (GLP-1(7-37)), Glucagon-like peptide 1(7-36) (GLP-1(7-36)), Glucagon-like peptide 2 (GLP-2)]
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基因ID
- 2641
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UniProt
- P01275
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