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GLP-1 ELISA 试剂盒

Name: GLP-1 ELISA 试剂盒
SKU: ABIN8068911
Availability: OutOfStock

This Colorimetric ELISA kit is designed for the quantitative measurement of 人, 小鼠和大鼠 GLP-1.

产品编号 ABIN8068911

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GLP-1 ELISA 试剂盒 (ABIN8068911)

抗原

See all GLP-1 ELISA试剂盒

GLP-1 (Glucagon-like peptide 1 (GLP-1))

抗原表位

total

适用

人, 小鼠, 大鼠

检测方法

Colorimetric

实验类型

Competition ELISA

检测范围

0.1 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum

Discover our top product GLP-1 ELISA Kit

最低检测浓度

0.1 pg/mL

原理

Human/Mouse/Rat total Glucagon-like peptide-1 EIA Kit for optimized for serum and cell culture medium. Competition-based ELISA

Analytical Method

Quantitative

特异性

This kit measures total GLP-1, including the full-length (1-37), active (7-36), and inactive (9-36) forms. The antibody shows similarly high affinity for each form. No cross-reactivity observed with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.

灵敏度

0.288 pg/mL

组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Lyophilized Standard
- Capture Antibody
- Assay Diluent
- Biotinylated Peptide
- HRP-Streptavidin
- Positive Control
- TMB One-Step Substrate Reagent
- Stop Solution
试剂未包括
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1-liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Software which can perform four-parameter logistic regression models
- Tubes to prepare standard or sample dilutions
- Orbital shaker
- Aluminum foil
- Plastic wrap
Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum

Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum

Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum

Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate

Glucagon-like peptide 1 (GLP-1) ELISA Kit
Small Sample GLP-1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum

Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: 人 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum

Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: 人 AA 7-36 Colorimetric Sandwich ELISA 0.06-54 pM/L Plasma

应用备注

Suggested Plasma Dilution: Human: 2X / Mouse: 2X / Rat: 2X

样本量

100 μL

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL Capture Antibody to each well. Incubate 1.5 hours at room temperature or overnight at 4 °C.
3. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C.
4. Add 100 μL prepared Streptavidin solution. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.
抗原 See all GLP-1 ELISA试剂盒

GLP-1 (Glucagon-like peptide 1 (GLP-1))

别名

GLP-1

背景

Gene Symbols: GCG
Background: Glucagon-like peptide-1 (GLP-1) is a highly conserved amino-acid-long peptide hormone among mammals and mainly secreted from the intestinal L-cells and the CNS. Functionally, GLP-1 plays an important role in energy homeostasis and nutrient absorption. The initial product GLP-1 (1-37) is susceptible to amidation and proteolytic cleavage, which gives rise to the two truncated and equipotent biologically active forms, GLP-1 (7-36) amide and GLP-1 (7-37). Then, the active forms of GLP-1 are extremely susceptible to the catalytic activity of the proteolytic enzyme dipeptidyl peptidase-4 (DPP-4), resulting in the abundant inactivated GLP-1 (9-36) amide constituting 60-80 % of total GLP-1 in circulation. This kit measures total GLP-1 including full length GLP-1 (1-37), the active (7-36) and inactive (9-36) forms of GLP-1.
Protein Name (Synonyms): Pro-glucagon [Cleaved into: Glicentin, Glicentin-related polypeptide (GRPP), Oxyntomodulin (OXM) (OXY), Glucagon, Glucagon-like peptide 1 (GLP-1) (Incretin hormone), Glucagon-like peptide 1(7-37) (GLP-1(7-37)), Glucagon-like peptide 1(7-36) (GLP-1(7-36)), Glucagon-like peptide 2 (GLP-2)]

基因ID

2641

UniProt

P01275