GLP-1 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for GLP-1 ELISA 试剂盒 (ABIN8068910)
抗原
See all GLP-1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 0.01 pg/mL
-
原理
- This kit specifically measures the inactive GLP-1 forms (9-36 and 9-37 amide), and does not detect the original (1-37 amide) or active (7-36 and 7-37 amide) forms.
-
Analytical Method
- Quantitative
-
特异性
- This EIA kit detects GLP-1 (ZGLP1)
-
灵敏度
- 0.663 pg/mL
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Assay Diluent
- Lyophilized Standard
- Positive Control
- Capture Antibody
- HRP-conjugated secondary antibody
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
-
试剂未包括
-
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1-liter graduated cylinders
- Distilled or deionized water
- Tubes to prepare standard or sample dilutions
- Orbital shaker
- Software which can perform four-parameter logistic regression models
-
-
Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitSmall Sample GLP-1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 AA 7-36 Colorimetric Sandwich ELISA 0.06-54 pM/L Plasma
-
-
应用备注
- Suggested Plasma Dilution: 2-4 folds
-
样本量
- 50 μL
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed.
- Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
- Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
- Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 5-20 minutes at room temperature or 37 °C.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.
-
-
- GLP-1 (Glucagon-like peptide 1 (GLP-1))
-
别名
- GLP-1
-
背景
-
Gene Symbols: GCG
Background: Glucagon-like peptide-1 (GLP-1) is a highly conserved amino-acid-long peptide hormone among mammals and mainly secreted from the intestinal L-cells and the CNS. Functionally, GLP-1 plays an important role in energy homeostasis and nutrient absorption. The initial product GLP-1 (1-37) is susceptible to amidation and proteolytic cleavage, which gives rise to the two truncated or equipotent biologically active forms, GLP-1 (7-36) and GLP-1 (7-37) amide. Then, the inactive forms of GLP-1 are extremely susceptible to the catalytic activity of the proteolytic enzyme dipeptidyl peptidase-4 (DPP-4), resulting in the abundant inactivated GLP-1 (9-36) or GLP-1 (9-37) amide constituting 60-80 % of total GLP-1 in circulation. This kit could specifically measure the inactive (9-36 and 9-37 amide) GLP-1, while not the original (1-37 amide) and active (7-36 and 7-37 amide) forms of GLP-1.
The inactive GLP-1 Enzyme Immunoassay (EIA) Kit (EIA-AGLP1) is a competitive enzyme immunoassay developed for rapid detection and quantitation of inactive GLP-1 in serum and plasma samples collected from various species including humans, porcine, feline, canine, rabbit, equine and sheep, but not rats or mice. The quantity of inactive GLP-1 is determined by a known standard GLP-1 curve. The inactive GLP-1 kit has a high sensitivity of approximately 0.663 pg/mL. The performance was also verified to exhibit good characteristics in terms of linearity, stability, precision, and spiking & recovery. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
Protein Name (Synonyms): Pro-glucagon [Cleaved into: Glicentin, Glicentin-related polypeptide (GRPP), Oxyntomodulin (OXM) (OXY), Glucagon, Glucagon-like peptide 1 (GLP-1) (Incretin hormone), Glucagon-like peptide 1(7-37) (GLP-1(7-37)), Glucagon-like peptide 1(7-36) (GLP-1(7-36)), Glucagon-like peptide 2 (GLP-2)]
-
基因ID
- 2641
-
UniProt
- P01275
抗原 See all GLP-1 ELISA试剂盒
-
