Fibrinogen ELISA 试剂盒
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北京 101111
Quick Overview for Fibrinogen ELISA 试剂盒 (ABIN8068828)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.5625 ng/mL
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原理
- Citrullinated Fibrinogen Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Citrullinated Fibrinogen (Fgn).
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects human Citrullinated Fibrinogen.
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灵敏度
- 0.19 ng/mL
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组件
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- Size / Qty
- Anti-Pan-Fgn Microplate
- 96 wells
- Citrullinated-Fgn Standard
- Anti-Citrullinated Fgn Detection Antibody
- HRP-conjugated anti-mouse IgG concentrate
- 25 μL
- Wash Buffer
- Assay Diluent B
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare positive control or sample dilutions
- Distilled or deionized water
- Shaker
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Fibrinogen ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Fibrinogen ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma
Fibrinogen ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 0.312 μg/mL - 20 μg/mL Plasma, Serum, Tissue Homogenate
Fibrinogen ELISA Kit适用: Cow Colorimetric Competition ELISA 3.125 μg/mL - 200 μg/mL Plasma, Serum
Fibrinogen ELISA Kit适用: 犬 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Fibrinogen ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 15.625 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Fibrinogen ELISA Kit适用: 山羊 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Fibrinogen ELISA Kit适用: 人 Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
Fibrinogen ELISA Kit适用: Pig Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
Fibrinogen ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 25 ng/mL - 800 ng/mL Plasma, Serum, Urine
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL of each standard and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-conjugated anti-mouse IgG solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- Fibrinogen
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背景
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Gene Symbols: FGB
Background: The Citrullinated Fibrinogen Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Citrullinated Fibrinogen (Fgn). An anti-pan fibrinogen antibody has been coated onto a 96-well plate. Standard and Samples are pipetted into the wells and fibrinogen present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-citrullinated fibrinogen antibody is used to detect Citrullinated Fibrinogen. After washing away unbound antibody, HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Citrullinated Fibrinogen bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Fibrinogen beta chain [Cleaved into: Fibrinopeptide B, Fibrinogen beta chain]
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基因ID
- 2244
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UniProt
- P02675
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