Estradiol ELISA 试剂盒
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Quick Overview for Estradiol ELISA 试剂盒 (ABIN8068771)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.01 pg/mL
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原理
- Estradiol Enzyme Immunoassay Kit is a competitive enzyme immunoassay developed for rapid E2 detection within 2.5 hours in serum and plasma samples collected from various species.
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Analytical Method
- Quantitative
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灵敏度
- 0.09 pg/mL
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组件
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- Pre-Coated 96-well Strip Microplate
- Assay Diluent
- Lyophilized Standard
- Positive Control
- Capture Antibody
- HRP-conjugated secondary antibody
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1-liter graduated cylinders
- Distilled or deionized water
- Tubes to prepare standard or sample dilutions
- Orbital shaker
- Software which can perform four-parameter logistic regression models
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Estradiol ELISA Kit
适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estradiol ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Estradiol ELISA Kit适用: Cow Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Estradiol ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Estradiol ELISA KitSmall Sample 适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estradiol ELISA KitQuick 适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estradiol ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 0.7 ng/mL - 45 ng/mL Plasma, Serum, Tissue Homogenate
Estradiol ELISA Kit适用: Various Species Colorimetric Sandwich ELISA Fecal, Tissue Culture Medium, Urine
Estradiol ELISA Kit适用: 绵羊 Colorimetric Competition ELISA 40-1400 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 2-3 folds
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样本量
- 50 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
- Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
- Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 5-20 minutes at room temperature or 37 °C.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.
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- Estradiol
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物质类
- Hormone
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背景
- Optional[Gene Symbols]: Estradiol (E2) is an estrogen steroid hormone, also known as 17β-estradiol (to distinguish it from 17α-estradiol) or as estra-1,3,5(10)-triene-3,17β-diol. It is the major female sex hormone, and involved in the regulation of the estrous and menstrual female reproductive cycles. Estradiol is responsible for the female sexual development, and is important in the development and maintenance of female reproductive tissues. Estradiol is produced mainly by the granulosa cells of the ovaries, is also in other tissues including the testicles, the adrenal glands, fat, liver, the breasts, and the brain.
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