dsDNA ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 dsDNA.
产品编号 ABIN8068677
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for dsDNA ELISA 试剂盒 (ABIN8068677)
抗原
See all dsDNA products
dsDNA
(Double-Stranded DNA (dsDNA))
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
检测范围
1.37 units/mL - 1000 units/mL
应用范围
ELISA
样品类型
Plasma, Serum
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最低检测浓度
- 1.37 units/mL
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原理
- The Double Stranded DNA (dsDNA) Human IgG ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgG antibody against pathogen antigen(s) in human serum or plasma.
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Analytical Method
- Quantitative
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组件
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- Size / Description
- dsDNA Coated 96-Well Microplate
- 96 wells coated with dsDNA
- Wash Buffer
- Positive Control
- Biotinylated Anti-Human IgG
- HRP-Streptavidin
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent
- Sample Diluent
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
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应用备注
- Suggested Plasma Dilution: 500-fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control, or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into respective wells. Incubate 1 hour at room temperature.
- Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- dsDNA (Double-Stranded DNA (dsDNA))
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别名
- dsDNA
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物质类
- Nucleotide
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背景
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Optional[Gene Symbols]: The Double Stranded DNA (dsDNA) Human IgG ELISA Kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with a pathogen antigen, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgG antibody is added, a complex forms between biotinylated secondary antibody and human antibody bound to the dsDNA coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-pathogen antigen antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Control consists of an anti-Double Stranded DNA (dsDNA) antibody. A biotinylated anti-Positive Control antibody is added to the Positive Control wells and treated identically to the samples. The intensity of the resulting color (measured at 450 nm) is used as a calibration curve for interpretation purposes in the different assays.
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