Digoxin ELISA 试剂盒
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Quick Overview for Digoxin ELISA 试剂盒 (ABIN8068666)
抗原
See all Digoxin (DIG) products适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.01 ng/mL
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原理
- This kit is a competitive immunoassay that quantifies DIG in serum or plasma from human, mouse, rat, equine, and bovine samples, as well as urine samples within 2.5 hours.
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Analytical Method
- Quantitative
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特异性
- This EIA kit detects Digoxin
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灵敏度
- 0.0427 ng/mL
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组件
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- Size / Description
- DIG Microplate
- 96 wells coated with DIG
- Assay Diluent
- Standard DIG
- Positive Control
- Anti-DIG Antibody
- ~10 μL
- HRP-conjugated Secondary Antibody
- ~5 μL
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1-liter graduated cylinders
- Distilled or deionized water
- Tubes to prepare standard or sample dilutions
- Orbital shaker
- GraphPad Prism or SigmaPlot software
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Digoxin (DIG) CLIA Kit
DIG 适用: 人 Chemiluminescent Competition ELISA
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应用备注
- Suggested Plasma Dilution: 2-4 folds
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样本量
- 50 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
- Add 50 μL anti-DIG to appropriate well. Incubate 1 hour at room temperature. And wash 5 times.
- Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature. And wash 5 times.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10-25 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.
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- Digoxin (DIG)
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别名
- Digoxin
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物质类
- Chemical
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背景
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Optional[Gene Symbols]: Digoxin (DIG) is a once-daily medication used to treat certain heart conditions when first-line therapies are ineffective or when additional treatment is required. Because digoxin has a narrow therapeutic index, careful monitoring is essential. Excessive digoxin levels can lead to dangerous, and sometimes fatal, cardiac arrhythmias. Therefore, serum digoxin testing helps ensure that drug levels are sufficient to provide therapeutic benefit while avoiding toxicity.
The DIG Enzyme Immunoassay (EIA) Kit is a competitive immunoassay that quantifies DIG in serum or plasma from human, mouse, rat, equine, and bovine samples, as well as urine samples within 2.5 hours. Absorbance is measured at 450 nm, and concentrations are interpolated from a ready-to-use DIG standard curve. The assay demonstrates a sensitivity of 0.0427 ng/mL and has been validated for linearity, stability, precision, and spike-recovery performance. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
抗原 See all Digoxin (DIG) products
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