Cyclic GMP ELISA 试剂盒
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Quick Overview for Cyclic GMP ELISA 试剂盒 (ABIN8068547)
抗原
See all Cyclic GMP (cGMP) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.01 pmol/mL
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原理
- Cyclic GMP (cGMP) Enzyme Immunoassay (EIA) Kit is a competitive enzyme immunoassay developed for rapid cGMP detection within 2.5 hours in serum and plasma samples collected from various species including humans, porcine, feline, canine, rabbit, equine and sheep, but not rats or mice.
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Analytical Method
- Quantitative
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特异性
- This EIA kit detects cGMP / Cyclic GMP
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组件
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- Pre-Coated 96-well Strip Microplate
- Assay Diluent
- Lyophilized Standard
- Positive Control
- Capture Antibody
- HRP-conjugated secondary antibody
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1-liter graduated cylinders
- Distilled or deionized water
- Tubes to prepare standard or sample dilutions
- Orbital shaker
- Software which can perform four-parameter logistic regression models
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Cyclic GMP (cGMP) ELISA Kit
cGMP 适用: Various Species Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cyclic GMP (cGMP) ELISA KitSmall Sample cGMP 适用: Various Species Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 2-3 folds
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样本量
- 50 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
- Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
- Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 5-20 minutes at room temperature or 37 °C.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.
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- Cyclic GMP (cGMP)
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别名
- cGMP
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物质类
- Chemical
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背景
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Optional[Gene Symbols]: Cyclic guanosine monophosphate (cGMP) is a cyclic nucleotide derived from GTP by guanylate cyclase and acts as a second messenger much like cyclic AMP. Some hormones, such as acetylcholine, insulin, oxytocin, serotonin, and histamine as well as vasodilators, including niroprusside, nitroglycerin, sodium nitrate, atrial natriuretic peptide (ANP), and nitric oxide (NO) could activate guanylate cyclases and stimulate cGMP levels. Through protein kinases activation, accumulated cGMP can relax smooth muscle and proliferation, phototransduction, and energy homeostasis. cGMP concentration in urine can be measured for kidney function and diabetes detection.
The Cyclic GMP (cGMP) Enzyme Immunoassay (EIA) Kit (EIA-CGMP) is a competitive enzyme immunoassay developed for rapid CGMP detection within 2.5 hours in serum and plasma samples collected from various species including humans, porcine, feline, canine, rabbit, equine and sheep, but not rats or mice. Absorbance is read at 450 nm. The quantity of CGMP is determined by a known standard CGMP curve. The CGMP kit has a high sensitivity of approximately 0.057 pmol/mL. The performance was also verified to exhibit good characteristics in terms of linearity, stability, precision, and spiking & recovery. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
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