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CRYAB ELISA 试剂盒

Name: CRYAB ELISA 试剂盒
SKU: ABIN8068533
Availability: OutOfStock

This Colorimetric ELISA kit is designed for the quantitative measurement of 人 CRYAB.

产品编号 ABIN8068533

发货至: 中国

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中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CRYAB ELISA 试剂盒 (ABIN8068533)

抗原

See all CRYAB ELISA试剂盒

CRYAB (Crystallin, alpha B (CRYAB))

适用

人

检测方法

Colorimetric

实验类型

Indirect ELISA

应用范围

ELISA

样品类型

Plasma, Serum

Discover our top product CRYAB ELISA Kit

原理

Alpha-B crystallin (CRYAB) Human IgA ELISA Kit is an in-vitro indirect ELISA for the quantitative measurement of Human IgA autoantibody against CRYAB in serum or plasma samples. This product is for research use only. Not for therapeutic or diagnostic applications.

Analytical Method

Quantitative

组件
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- Sample Diluent
试剂未包括
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
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应用备注

Optimal working dilution should be determined by the investigator.

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
抗原 See all CRYAB ELISA试剂盒

CRYAB (Crystallin, alpha B (CRYAB))

别名

CRYAB

背景

Gene Symbols: CRYAB CRYA2 HSPB5
Protein Name (Synonyms): Alpha-crystallin B chain (Alpha(B)-crystallin) (Heat shock protein beta-5) (HspB5) (Renal carcinoma antigen NY-REN-27) (Rosenthal fiber component)

基因ID

1410

UniProt

P02511