Cortisol ELISA 试剂盒
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北京 101111
Quick Overview for Cortisol ELISA 试剂盒 (ABIN8068527)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.1 ng/mL
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原理
- Human/Mouse/Rat Cortisol EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA
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Analytical Method
- Quantitative
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特异性
- This EIA kit is designed to detect human, mouse, and rat Cortisol.
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灵敏度
- 2.7 ng/mL
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组件
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- Pre-Coated 96-well Strip Microplate
- Standard Peptide
- HRP-Conjugated Peptide
- Capture Antibody
- Positive Control
- Wash Buffer
- Assay Diluent(s)
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Distilled or deionized water
- Aluminum foil
- Plastic wrap
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Cortisol ELISA Kit
适用: Various Species Colorimetric Sandwich ELISA Fecal, Hair, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Culture Medium, Urine
Cortisol ELISA Kit适用: Various Species Colorimetric Sandwich ELISA 100 pg/mL - 3200 pg/mL Fecal, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Culture Medium, Urine
Cortisol ELISA Kit适用: Various Species Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Urine
Cortisol ELISA KitQuick 适用: 人 Colorimetric Competition ELISA 6.25 ng/mL - 400 ng/mL Plasma, Saliva, Serum, Urine
Cortisol ELISA KitQuick 适用: 猴 Colorimetric Competition ELISA 3.13 ng/mL - 200 ng/mL Plasma, Serum
Cortisol ELISA KitQuick 适用: Pig Colorimetric Competition ELISA 3.13 ng/mL - 200 ng/mL Plasma, Serum
Cortisol ELISA KitQuick 适用: 兔 Colorimetric Competition ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Cortisol ELISA KitQuick 适用: 绵羊 Colorimetric Competition ELISA 3.13 ng/mL - 200 ng/mL Plasma, Serum
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应用备注
- Suggested Plasma Dilution: Human: 8x / Mouse: 2x / Rat: 2x
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well. Incubate 1.5 hours at room temperature or overnight at 4 °C.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read plate at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.
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- Cortisol
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物质类
- Hormone
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背景
- Optional[Gene Symbols]: Cortisol is a naturally occurring pregnane corticosteroid and is also known as 11β,17α,21-trihydroxypregn-4-ene-3,20-dione. Cortisol is synthesized from cholesterol mainlyby the adrenal gland in the zona fasciculata. Release of Cortisolis increased in response to stress and low blood-glucose concentration. It functions to increase blood sugar through gluconeogenesis, to suppress the immune system, and to aid in the metabolism of fat, protein, and carbohydrates. It also decreases bone formation.
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