CRH ELISA 试剂盒
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北京 101111
Quick Overview for CRH ELISA 试剂盒 (ABIN8068525)
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See all CRH ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.016 ng/mL
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原理
- Competitive ELISA (EIA) kit for detecting Corticotropin Releasing Factor (CRF), also known as Corticotropin Releasing Hormone (CRH), in serum and cell culture medium samples.
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Analytical Method
- Quantitative
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特异性
- This EIA kit is designed to detect human, mouse, and rat Corticotropin Releasing Factor
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灵敏度
- 0.1 ng/mL
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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Corticotropin Releasing Hormone (CRH) ELISA Kit
CRH 适用: 小鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Corticotropin Releasing Hormone (CRH) ELISA KitCRH 适用: 小鼠, 大鼠 Colorimetric Sandwich ELISA
Corticotropin Releasing Hormone (CRH) ELISA KitCRH 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Corticotropin Releasing Hormone (CRH) ELISA KitCRH 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Corticotropin Releasing Hormone (CRH) ELISA KitCRH 适用: 大鼠 Colorimetric Competition ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Corticotropin Releasing Hormone (CRH) ELISA KitCRH 适用: 绵羊 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Corticotropin Releasing Hormone (CRH) ELISA KitCRH 适用: 人 Colorimetric Competition ELISA 0.313 pg/mL - 20 pg/mL Plasma, Serum, Tissue Homogenate
Corticotropin Releasing Hormone (CRH) ELISA KitCRH 适用: 小鼠 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Corticotropin Releasing Hormone (CRH) ELISA KitCRH 适用: Pig Colorimetric Competition ELISA 1.563 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Corticotropin Releasing Hormone (CRH) ELISA KitCRH 适用: 大鼠 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: Human: 4X / Mouse: 2X / Rat: 2X
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Standard, Biotinylated CRF / CRH peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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- CRH (Corticotropin Releasing Hormone (CRH))
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别名
- Corticotropin Releasing Factor
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背景
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Gene Symbols: CRH
Background: Corticotropin-releasing factor (CRF) (also known as Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Its main function is the stimulation of the pituitary synthesis of adrenocorticotropic hormone (ACTH), as part of the hypothalamic-pituitary-adrenal axis (HPA axis). CRF levels may be associated with Alzheimer"s disease and major depression. CRF is also synthesized in peripheral tissues, such as T lymphocytes, and is highly expressed in the placenta, suggesting roles in parturition.
The RayBiotech CRF Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting CRF peptide based on the competitive enzyme immunoassay principle. In this assay, a biotinylated CRF peptide is spiked into the samples and standards. The samples and standards are then added to the plate, where the biotinylated CRF peptide competes with endogenous (unlabeled) CRF for binding to the anti-CRF antibody. After a wash step, any bound biotinylated CRF then interacts with horseradish peroxidase (HRP)-streptavidin, which catalyzes a color development reaction. The intensity of the colorimetric signal is directly proportional to the amount of captured biotinylated CRF peptide and inversely proportional to the amount of endogenous CRF in the standard or samples. A standard curve of known concentration of CRF peptide can be established and the concentration of CRF peptide in the samples can be calculated accordingly.Protein Name (Synonyms): Corticotropin-releasing hormone (CRH), corticotropin-releasing factor (CRF), corticoliberin
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基因ID
- 1392
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UniProt
- P06850
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途径
- Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour
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