Corticosterone ELISA 试剂盒
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Quick Overview for Corticosterone ELISA 试剂盒 (ABIN8068521)
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See all Corticosterone (CORT) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.0001 ng/mL
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原理
- This kit is a competitive immunoassay that quantifies corticosterone in serum or plasma from human, porcine, feline, canine, rabbit, equine, and sheep samples (not rat or mouse) within 2.5 hours.
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Analytical Method
- Quantitative
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特异性
- This EIA kit detects Corticosterone
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灵敏度
- 0.0021 ng/mL
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组件
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- Pre-Coated 96-well Strip Microplate
- Assay Diluent
- Lyophilized Standard
- Positive Control
- Capture Antibody
- HRP-conjugated secondary antibody
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1-liter graduated cylinders
- Distilled or deionized water
- Tubes to prepare standard or sample dilutions
- Orbital shaker
- Software which can perform four-parameter logistic regression models
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Corticosterone (CORT) ELISA Kit
CORT 适用: Various Species Colorimetric Sandwich ELISA Fecal, Plasma (EDTA), Plasma (heparin), Serum, Tissue Culture Medium, Urine
Corticosterone (CORT) ELISA KitCORT 适用: Various Species Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Plasma, Serum, Urine
Corticosterone (CORT) ELISA KitCORT 适用: 人 Colorimetric Competition ELISA Plasma
Corticosterone (CORT) ELISA KitCORT 适用: 大鼠 Colorimetric Competition ELISA 2.813 ng/mL - 180 ng/mL Plasma, Serum, Tissue Homogenate
Corticosterone (CORT) ELISA KitCORT 适用: Various Species Colorimetric Competition ELISA 2.813 ng/mL - 180 ng/mL Plasma, Serum, Tissue Homogenate
Corticosterone (CORT) ELISA KitCORT 适用: 小鸡 Colorimetric Competition ELISA 2.813 ng/mL - 180 ng/mL Plasma, Serum, Tissue Homogenate
Corticosterone (CORT) ELISA KitCORT 适用: 大鼠 Colorimetric Competition ELISA 0.2-40 ng/mL Plasma, Serum, Tissue Homogenate
Corticosterone (CORT) ELISA KitCORT 适用: 人 Colorimetric Competition ELISA 2.813 ng/mL - 180 ng/mL Plasma, Serum, Tissue Homogenate
Corticosterone (CORT) ELISA KitCORT 适用: 兔 Colorimetric Competition ELISA 2.813 ng/mL - 180 ng/mL Plasma, Serum, Tissue Homogenate
Corticosterone (CORT) ELISA KitCORT 适用: 小鼠 Colorimetric Competition ELISA 2.813 ng/mL - 180 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 2-4 folds
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样本量
- 50 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
- Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
- Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 5-20 minutes at room temperature or 37 °C.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.
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- Corticosterone (CORT)
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别名
- Corticosterone
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物质类
- Hormone
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背景
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Optional[Gene Symbols]: Corticosterone, a glucocorticoid synthesized in the adrenal cortex upon ACTH stimulation, serves as both the biosynthetic precursor of aldosterone and a key biomarker of stress. Stress-induced elevations in corticosterone have been linked to impaired long-term memory retrieval, chronic increases following dietary restriction, and marked rises after burn injury. Beyond its role in the stress response, corticosterone also helps shape sleep-wake cycles.
The Corticosterone Enzyme Immunoassay (EIA) Kit (EIA-CORT2) is a competitive immunoassay that quantifies corticosterone in serum or plasma from human, porcine, feline, canine, rabbit, equine, and sheep samples (not rat or mouse) within 2.5 hours. Absorbance is measured at 450 nm and values are interpolated from a ready-to-use corticosterone standard curve. The assay demonstrates a sensitivity of 0.0021 ng/mL and has been validated for linearity, stability, precision, and spike-recovery performance. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
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