CXCL10 ELISA 试剂盒
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Quick Overview for CXCL10 ELISA 试剂盒 (ABIN8068374)
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See all CXCL10 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 7.26 pg/mL
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原理
- The Rat IP-10 (CXCL10) ELISA Kit is a pre-coated sandwich-based assay kit designed to quantitatively measure IP-10 (CXCL10) levels in rat cell culture supernatants, plasma, and serum.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects rat IP-10. Other species not determined.
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组件
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- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
- Adhesive Sealing Film
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard or sample dilutions
- Plastic wrap
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Chemokine (C-X-C Motif) Ligand 10 (CXCL10) ELISA Kit
CXCL10 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 10 (CXCL10) ELISA KitCXCL10 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 10 (CXCL10) ELISA KitCXCL10 适用: 人 AA 22-98 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Chemokine (C-X-C Motif) Ligand 10 (CXCL10) ELISA KitCXCL10 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 10 (CXCL10) ELISA KitCXCL10 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 10 (CXCL10) ELISA KitCXCL10 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 10 (CXCL10) ELISA KitCXCL10 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 10 (CXCL10) ELISA KitCXCL10 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 10 (CXCL10) ELISA KitCXCL10 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 10 (CXCL10) ELISA KitCXCL10 适用: 小鼠 AA 22-98 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
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应用备注
- Suggested Plasma Dilution: 5 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
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别名
- IP-10
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背景
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Gene Symbols: CXCL10
Protein Name (Synonyms): C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Interferon-inducible protein 10) (Protein Mob-1) (Small-inducible cytokine B10)
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UniProt
- P48973
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