Cadherin 5 ELISA 试剂盒
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Quick Overview for Cadherin 5 ELISA 试剂盒 (ABIN8068127)
抗原
See all Cadherin 5 (CDH5) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.66 ng/mL
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原理
- Human VE-Cadherin (CDH5) ELISA Kit for Cell Culture Supernatants and Serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human ALCAM (CD166), BCAM, CEACAM-1, E-Cadherin, TROP1 (EpCAM), E-Selectin, ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), L-Selectin (CD62L), NCAM-1 (CD56), NrCAM, P-Cadherin, P-Selectin, PECAM-1 (CD31), VCAM-1 (CD106).
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Cadherin 5 (CDH5) ELISA Kit
CDH5 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 大鼠 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 人 AA 48-599 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 小鼠 AA 46-599 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 人 Colorimetric Sandwich ELISA 3.9-250 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- Cadherin 5 (CDH5)
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别名
- VE-Cadherin
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背景
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Gene Symbols: CDH5
Protein Name (Synonyms): Cadherin-5 (7B4 antigen) (Vascular endothelial cadherin) (VE-cadherin) (CD antigen CD144)
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UniProt
- P33151
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途径
- Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2
抗原 See all Cadherin 5 (CDH5) ELISA试剂盒
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