AGA ELISA 试剂盒
The 人 AGA ELISA Kit (ABIN8068024) is a Colorimetric ELISA Kit designed to quantify 人 AGA.
产品编号 ABIN8068024
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for AGA ELISA 试剂盒 (ABIN8068024)
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See all AGA ELISA试剂盒
AGA
(Aspartylglucosaminidase (AGA))
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
检测范围
1.37 units/mL - 1000 units/mL
应用范围
ELISA
样品类型
Plasma, Serum
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最低检测浓度
- 1.37 units/mL
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原理
- The Aspartylglucosaminidase (AGA) Human IgG ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgG autoantibodies against AGA in human serum or plasma.
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Analytical Method
- Quantitative
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组件
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- Size / Description
- AGA Coated 96-Well Microplate
- 96 wells coated with AGA
- Wash Buffer
- Positive Control
- Negative Serum Control
- Biotinylated Anti-Human IgG
- HRP-Streptavidin
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent
- Sample Diluent
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
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应用备注
- Suggested Plasma Dilution: 500-fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control, negative control, or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into respective wells. Incubate 1 hour at room temperature.
- Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
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- AGA (Aspartylglucosaminidase (AGA))
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别名
- Aspartylglucosaminidase
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背景
- Optional[Gene Symbols]: The Aspartylglucosaminidase (AGA) Human IgG ELISA Kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with AGA protein, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgG antibody is added, a complex forms between biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-AGA antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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