WNV IgG ELISA 试剂盒
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北京 101111
Quick Overview for WNV IgG ELISA 试剂盒 (ABIN8067980)
抗原
See all WNV IgG (WMV IgG) products适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.37 units/mL
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原理
- The West Nile Virus (Lineage 1) E Protein Human IgG ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgG antibody against WNV1E antigen(s) in human serum or plasma.
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Analytical Method
- Semi-Quantitative
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组件
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- Size / Description
- WNV1E Coated 96-Well Microplate
- 96 wells coated with WNV1E
- Wash Buffer
- Positive Control
- Biotinylated Anti-Human IgG
- HRP-Streptavidin
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent
- Sample Diluent
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
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应用备注
- Suggested Plasma Dilution: 1,000-fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control, or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into respective wells. Incubate 1 hour at room temperature.
- Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The unopened kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles.
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- WNV IgG (WMV IgG) (Anti-West Nile Virus IgG (WMV IgG))
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别名
- West Nile Virus IgG
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物质类
- Antibody
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背景
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Gene Symbols: IgG
Background: The West Nile Virus (Lineage 1) E Protein Human IgG ELISA Kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with WNV1E protein, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgG antibody is added, a complex forms between biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-WNV1E antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): IgG, West Nile Virus (Lineage 1) Envelope Protein (E)
抗原 See all WNV IgG (WMV IgG) products
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