ANGPTL4 ELISA 试剂盒
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Quick Overview for ANGPTL4 ELISA 试剂盒 (ABIN8067835)
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See all ANGPTL4 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.75 ng/mL
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原理
- Mouse ANGPTL4 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects mouse ANGPTL4. Other species not determined.
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Angiopoietin-Like 4 (ANGPTL4) ELISA Kit
ANGPTL4 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 小鼠 Colorimetric Competition ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 人 AA 165-406 Colorimetric Sandwich ELISA 625 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))
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别名
- Angiopoietin-like protein 4
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背景
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Gene Symbols: Angptl4
Protein Name (Synonyms): Angiopoietin-related protein 4 (425O18-1) (Angiopoietin-like protein 4) (Fasting-induced adipose factor) (Hepatic fibrinogen/angiopoietin-related protein) (HFARP) (Secreted protein Bk89) [Cleaved into: ANGPTL4 N-terminal cha
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UniProt
- Q9Z1P8
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途径
- Regulation of Lipid Metabolism by PPARalpha
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