5-Hydroxymethylcytosine ELISA 试剂盒
Cow, 马, 人, 小鼠, Pig, 大鼠 和 绵羊 5-Hydroxymethylcytosine ELISA Kit, Colorimetric is an assay for quantification of Cow, 马, 人, 小鼠, Pig, 大鼠 和 绵羊 5-Hydroxymethylcytosine.
产品编号 ABIN8067734
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Quick Overview for 5-Hydroxymethylcytosine ELISA 试剂盒 (ABIN8067734)
抗原
See all 5-Hydroxymethylcytosine (5-hmC) products
5-Hydroxymethylcytosine (5-hmC)
适用
Cow, 马, 人, 小鼠, Pig, 大鼠, 绵羊
检测方法
Colorimetric
实验类型
Competition ELISA
检测范围
0.1 pg/mL - 100000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma, Serum, Urine
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最低检测浓度
- 0.1 pg/mL
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原理
- 5-hmC ELISA Kit is an in vitro quantitative assay for detecting 5-Hydroxymethylation based on the competitive enzyme immunoassay principle.
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Analytical Method
- Quantitative
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特异性
- This EIA kit detects 5-hmC / 5-Hydroxymethylcytosine
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灵敏度
- 1.006 pg/mL
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Lyophilized Standard
- Capture Antibody
- Assay Diluent
- Biotinylated Peptide
- HRP-Streptavidin
- Positive Control
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1-liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Software which can perform four-parameter logistic regression models
- Tubes to prepare standard or sample dilutions
- Orbital shaker
- Aluminum foil
- Plastic wrap
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应用备注
- Suggested Plasma Dilution: 2-4 folds
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL Capture Antibody to each well. Incubate 1.5 hours at room temperature or overnight at 4 °C.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C.
- Add 100 μL prepared Streptavidin solution. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.
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- 5-Hydroxymethylcytosine (5-hmC)
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别名
- 5-hmC
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物质类
- Chemical
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背景
- Optional[Gene Symbols]: 5-hydroxymethylcytosine (5-hmC), also known as the ""sixth base"" of DNA, is generated by the oxidation of 5-methylcytosine (5-mC) through the action of the ten-eleven translocation (TET) family of enzymes. 5-hmC is thought to be the DNA demethylation pathway, thereby playing a crucial part in gene regulation, chromatin structure and accessibility, recruiting or repelling transcription factors, and ultimately modulating gene expression. The presence of 5-hmC is particularly abundant in neuronal cells, suggesting a significant role in brain development and function. In addition, levels of 5-hmC undergo highly dynamic changes during development, differentiation and cancer. 5-hmC plays a critical role in developmental processes and dysregulation of 5-hmC has been associated with several human diseases.
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