CA242 ELISA 试剂盒
人 CA242 ELISA Kit, Colorimetric is an assay for quantification of 人 CA242.
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产品编号 ABIN8065412
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for CA242 ELISA 试剂盒 (ABIN8065412)
抗原
See all CA242 products
CA242
(Carbohydrate Antigen 242 (CA242))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
31.25 pg/mL – 2000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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最低检测浓度
- 31.25 pg/mL
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原理
- In vitro quantitative determination of ca242 concentrations in serum, plasma, cell culture supernatant
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Analytical Method
- Quantitative
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特异性
- Specifically recognize ca242, no obvious cross reaction with other analogues
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灵敏度
- 18.75 pg/mL
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组件
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- ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description -
试剂未包括
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator
- Automated plate washer
- Precision single and multi-channel pipette and disposable tips
- Clean tubes and Eppendorf tubes
- Deionized or distilled water
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Carbohydrate Antigen 242 (CA242) ELISA Kit
CA242 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL – 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Sandwich ELISA, Double Antibody
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样本量
- 100 μL
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实验时间
- 4 h
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板类型
- Pre-coated
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实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ca242 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti ca242 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ca242 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ca242 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
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实验精密度
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Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate. CV% = <6%
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.CV%= <7% -
限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 2-8°C (for sealed box), please do not freeze! See kit label for expiry date
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- CA242 (Carbohydrate Antigen 242 (CA242))
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别名
- CA242
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背景
- CA242(Pancreatic Carcinoma Markers-CA242)
抗原 See all CA242 products
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