CRP ELISA 试剂盒
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Quick Overview for CRP ELISA 试剂盒 (ABIN8057098)
抗原
See all CRP ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.13 pg/mL
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Analytical Method
- Quantitative
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特异性
- Hs-CRP
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组件
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- Plate
- Standard
- Diluent
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试剂未包括
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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C-Reactive Protein (CRP) ELISA Kit
High Sensitivity CRP 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate, Urine
C-Reactive Protein (CRP) ELISA KitCRP 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate, Urine
C-Reactive Protein (CRP) ELISA KitHigh Sensitivity CRP 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 小鸡 Colorimetric Sandwich ELISA 1.563 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: Pig Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 豚鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: Cow Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 兔 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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实验流程
- This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat CRP.
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- CRP (C-Reactive Protein (CRP))
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别名
- hs-CRP
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背景
- C-reactive protein (CRP) is an annular, pentameric protein. The physiological role of CRP is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via the C1Q complex. CRP is an acute phase protein in humans and is an important mediator of immune host defense. The acute phase response occurs in response to a wide range of acute and chronic inflammatory conditions such as bacterial, viral, or fungal infections, rheumatic and other inflammatory diseases, malignancy, and tissue injury and necrosis. These conditions cause release of IL-6 and other cytokines that trigger the synthesis of CRP and fibrinogen by the liver. CRP plays a role in innate immunity as an early defense system against infections. Monitoring of CRP levels in serum provides more detailed insights in several pathological situations such as inflammatory disease (including inflammatory bowel disease, Intestinal Lymphoma, Intestinal Tuberculosis and Behcet's Syndrome), cancer, diabetes, hypertension, cardiovascular disease, obstructive sleep apnea and so on.
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基因ID
- 25419
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NCBI登录号
- NM_017096
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UniProt
- P48199
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途径
- Carbohydrate Homeostasis
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