APOA2 ELISA 试剂盒
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北京 101111
Quick Overview for APOA2 ELISA 试剂盒 (ABIN8057089)
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See all APOA2 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.53 pg/mL
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Analytical Method
- Quantitative
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特异性
- Apo A2
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组件
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- Plate
- Standard
- Diluent
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试剂未包括
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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Apolipoprotein A-II (APOA2) ELISA Kit
APOA2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: 人 Colorimetric Sandwich ELISA 21.875 ng/mL - 1400 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: Pig Colorimetric Sandwich ELISA 4.69 μg/mL - 300 μg/mL Plasma, Serum
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: Pig Colorimetric Sandwich ELISA 4.69 μg/mL - 300 μg/mL Plasma, Serum
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: 人 Colorimetric Sandwich ELISA Plasma, Serum
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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实验流程
- This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Apo A2.
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- APOA2 (Apolipoprotein A-II (APOA2))
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别名
- Apo A2
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背景
- Apolipoprotein AII (Apo A2), the second most abundant protein after Apo A1 of human plasma high-density lipoproteins (HDL), is the most lipophilic of the exchangeable apolipoproteins. Apo A2 is synthesized by the liver and the small intestine and found in plasma as a monomer, dimer. The Apo A2 gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre peptide, a short N terminal domain and a C terminal domain composed of a variable number of lipid binding amphipathic helices. Apo A2 may stabilize HDL structure by its association with lipids, and affect the HDL metabolism. Role of Apo A2 in lipid metabolism and atherosclerosis show important clinical significance. Apo A2 levels have been associated with increased susceptibility to atherosclerosis, increased free fatty acid levels, increased body fat, and increased insulin resistance.
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基因ID
- 336
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NCBI登录号
- NM_001643
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UniProt
- P02652
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途径
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Negative Regulation of Transporter Activity, Lipid Metabolism
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