Creatine Kinase-MB Isoenzyme ELISA 试剂盒
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Quick Overview for Creatine Kinase-MB Isoenzyme ELISA 试剂盒 (ABIN8056744)
抗原
适用
检测方法
实验类型
检测范围
应用范围
样品类型
质量等级
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最低检测浓度
- 1.56 ng/mL
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原理
- Mini Samples ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)
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Analytical Method
- Quantitative
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特异性
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This assay has high sensitivity and excellent specificity for detection of Mini Samples Creatine Kinase MB Isoenzyme (CKMB).
No significant cross-reactivity or interference between Mini Samples Creatine Kinase MB Isoenzyme (CKMB) and analogues was observed. -
灵敏度
- < 0.6 ng/mL
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组件
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- Pre-coated, 96-well strip plate
- Plate sealer
- Standard
- Standard Diluent
- Detection Reagent A
- Assay Diluent A
- Detection Reagent B
- Assay Diluent B
- TMB Substrate
- Stop Solution
- Wash Buffer (30X)
- Instruction manual
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试剂未包括
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- Microplate reader with 450 ± 10nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.
- Deionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution
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样本量
- 25 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
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1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Creatine Kinase MB Isoenzyme (CKMB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Creatine Kinase MB Isoenzyme (CKMB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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限制
- 仅限研究用
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注意事项
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. -
储存条件
- 4 °C,-20 °C
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储存方法
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- For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
- For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit. - For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
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有效期
- 6 months
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- Creatine Kinase-MB Isoenzyme
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别名
- Creatine Kinase MB Isoenzyme
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背景
- CK2, CK-MB, Creatine Kinase,Muscle/Brain
抗原
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