AMH CLIA Kit
Our Local Distributor
北京 101111
Quick Overview for AMH CLIA Kit (ABIN8056021)
抗原
See all AMH CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- CLIA Kit for Anti-Mullerian Hormone (AMH)
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Analytical Method
- Quantitative
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特异性
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This assay has high sensitivity and excellent specificity for detection of Anti-Mullerian Hormone (AMH).
No significant cross-reactivity or interference between Anti-Mullerian Hormone (AMH) and analogues was observed. -
灵敏度
- < 107 pg/mL
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组件
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- Pre-coated, 96-well strip plate
- Plate sealer
- Standard
- Standard Diluent
- Detection Reagent A
- Assay Diluent A
- Detection Reagent B
- Assay Diluent B
- Substrate A
- Substrate B
- Wash Buffer (30X)
- Instruction manual
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试剂未包括
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- Luminometer capable of reading 96-well microplates with the following parameters: lag time 30.0secs; read time 1.0 sec/well
- Single or multi-channel pipettes with high precision and disposable tips.
- Microcentrifuge Tubes.
- Deionized or distilled water.
- Absorbent paper for blotting the microplate.
- Container for Wash Solution.
- 0.01mol/L (or 1×) Phosphate Buffered Saline(PBS), pH7.0-7.2
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Anti-Mullerian Hormone (AMH) ELISA Kit
AMH 适用: 人 Colorimetric Competition ELISA 937.5 pg/mL - 15000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 人 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 大鼠 Colorimetric Competition ELISA 61.73 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 小鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Plasma, Serum
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: Cow Colorimetric Competition ELISA 61.73 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: Pig Colorimetric Competition ELISA 1.88 ng/mL - 30 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 小鸡 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitWide Range AMH 适用: 大鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
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样本量
- 50 μL
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
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1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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限制
- 仅限研究用
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注意事项
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. -
储存条件
- 4 °C,-20 °C
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储存方法
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- For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
- For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit. - For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
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有效期
- 6 months
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- AMH (Anti-Mullerian Hormone (AMH))
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别名
- Anti-Mullerian Hormone
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背景
- MIF, MIH, MIS, Müllerian Inhibiting Factor, Müllerian Inhibiting Hormone, Müllerian Inhibiting Substance
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UniProt
- P49000
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途径
- Negative Regulation of Hormone Secretion
抗原 See all AMH CLIA Kits
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