IL12B ELISA 试剂盒
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Quick Overview for IL12B ELISA 试剂盒 (ABIN8055967)
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See all IL12B ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 46.9 pg/mL
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原理
- Sandwich Porcine IL-12/IL-23 p40 ELISA Kit to quantitate Porcine IL-12/IL-23 p40 in cell culture supernatants, serum, plasma(heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Interleukin 12 beta (IL12B) ELISA Kit
IL12B 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 马 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 人 AA 23-328 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 小鼠 AA 23-335 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 山羊 Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 豚鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 绵羊 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 大鼠 Colorimetric Sandwich ELISA 11.719 pg/mL - 750 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.7%, 7.0%, 2.3%
Inter-Assay Precision (CV%): 5.4%, 7.6%, 6.4% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- IL12B (Interleukin 12 beta (IL12B))
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别名
- IL12B
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背景
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Background: Subunit beta of interleukin 12 (IL12B), also known as interleukin 12B, or interleukin-12 subunit p40, is a subunit of human interleukin 12. It is mapped to 5q33.3. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease.
Gene Full Name: interleukin 12b
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基因ID
- 397076
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UniProt
- Q28268
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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