NPY ELISA 试剂盒
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Quick Overview for NPY ELISA 试剂盒 (ABIN8055940)
抗原
See all NPY ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312.0 pg/mL
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原理
- Sandwich Human Neuropeptide Y/NPY ELISA Kit to quantitate Human Neuropeptide Y/NPY in cell culture supernatants, tissue, serum, plasma(heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 30 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Neuropeptide Y (NPY) ELISA Kit
NPY 适用: 人 Colorimetric Sandwich ELISA 0.78 pg/mL - 50 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 大鼠 Colorimetric Competition ELISA 2.47 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 人 Colorimetric Competition ELISA 4.94 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 小鼠 Colorimetric Competition ELISA 2.47 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: Cow Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
Neuropeptide Y (NPY) ELISA KitNPY 适用: 小鼠 Colorimetric Sandwich ELISA 1.87 pg/mL - 120 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 人 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.1%, 3.8%, 4.1%
Inter-Assay Precision (CV%): 3.6%, 6.6%, 6.8% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- NPY (Neuropeptide Y (NPY))
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别名
- NPY
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背景
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Background: This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi.
Gene Full Name: Pro-neuropeptide Y
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基因ID
- 4852
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UniProt
- P01303
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途径
- Feeding Behaviour
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