LAIR2 ELISA 试剂盒
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Quick Overview for LAIR2 ELISA 试剂盒 (ABIN8055937)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 7.8 pg/mL
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原理
- Sandwich Human LAIR2 ELISA Kit to quantitate Human LAIR2 in cell culture supernatants, serum, plasma(heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 2 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Leukocyte-Associated Immunoglobulin-Like Receptor 2 (LAIR2) ELISA Kit
LAIR2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Leukocyte-Associated Immunoglobulin-Like Receptor 2 (LAIR2) ELISA KitLAIR2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Leukocyte-Associated Immunoglobulin-Like Receptor 2 (LAIR2) ELISA KitLAIR2 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.7%, 5.8%, 5.7%
Inter-Assay Precision (CV%): 5.1%, 3.7%, 7.8% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- LAIR2 (Leukocyte-Associated Immunoglobulin-Like Receptor 2 (LAIR2))
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别名
- LAIR2
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背景
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Background: Leukocyte-associated immunoglobulin-like receptor 2 is a protein that in humans is encoded by the LAIR2 gene. It was identified by its similarity to leukocyte-associated immunoglobulin-like receptor 1, a membrane-bound receptor that modulates innate immune response. The protein encoded by this locus is a soluble receptor that may play roles in both inhibition of collagen-induced platelet aggregation and vessel formation during placental implantation. This gene maps to a region of 19q13.4, termed the leukocyte receptor cluster, which contains 29 genes in the immunoglobulin superfamily. Alternatively spliced transcript variants have been described for this gene.
Gene Full Name: leukocyte associated immunoglobulin like receptor 2
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基因ID
- 3904
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UniProt
- Q6ISS4
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