MEP1A ELISA 试剂盒
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Quick Overview for MEP1A ELISA 试剂盒 (ABIN8055893)
抗原
See all MEP1A ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.56 ng/mL
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原理
- Sandwich Human MEP1A ELISA Kit to quantitate Human MEP1A in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: V22-Q601Capture antibody and Detection antibody: polyclonal antibody from goat, biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 50 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) ELISA Kit
MEP1A 适用: 人 Colorimetric Competition ELISA 49.4 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) ELISA KitMEP1A 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) ELISA KitMEP1A 适用: 小鼠 Colorimetric Competition ELISA 49.4 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) ELISA KitMEP1A 适用: 大鼠 Colorimetric Competition ELISA 49.4 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) ELISA KitMEP1A 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.1%, 4%, 5.4%
Inter-Assay Precision (CV%): 6.6%, 6.7%, 6.7% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))
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别名
- MEP1A
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背景
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Background: Meprin A subunit alpha also known as endopeptidase-2 or PABA peptide hydrolase is the alpha subunit of the meprin A enzyme that in humans is encoded by the MEP1A gene. Meprin A subunit alpha, encoded by the MEP1A gene, is a component of the meprin A metalloproteinase complex, which is involved in extracellular matrix remodeling and proteolysis. Meprin A is a zinc-dependent endopeptidase found predominantly in the kidneys, intestines, and other tissues. It plays a crucial role in various physiological processes, including inflammation, tissue repair, and the processing of bioactive peptides. The alpha subunit, specifically, is a part of the hetero-oligomeric enzyme complex, which combines with the beta subunit to form an active enzyme. Abnormalities in meprin A function have been associated with several pathological conditions, including kidney diseases and cancer.
Gene Full Name: meprin A subunit alpha
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基因ID
- 4224
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UniProt
- Q16819
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