Integrin alpha 1 ELISA 试剂盒
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Quick Overview for Integrin alpha 1 ELISA 试剂盒 (ABIN8055829)
抗原
See all Integrin alpha 1 (ITGA1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human CD49A ELISA Kit. Quantitate Human CD49A in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: CHO
Immunogen sequence: M1-P1141Capture antibody and Detection antibody: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 75 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Integrin alpha 1 (ITGA1) ELISA Kit
ITGA1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Integrin alpha 1 (ITGA1) ELISA KitITGA1 适用: 小鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Integrin alpha 1 (ITGA1) ELISA KitITGA1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 6.1%, 2.1%, 2.6%
Inter-Assay Precision (CV%): 3.9%, 5.8%, 3.5% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- Integrin alpha 1 (ITGA1)
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别名
- ITGA1
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背景
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Background: Integrin alpha-1 also CD49a is an integrin alpha subunit encoded in humans by the gene ITGA1. This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.
Gene Full Name: integrin subunit alpha 1
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基因ID
- 3672
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UniProt
- P56199
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途径
- EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex
抗原 See all Integrin alpha 1 (ITGA1) ELISA试剂盒
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