GSTM1 ELISA 试剂盒
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Quick Overview for GSTM1 ELISA 试剂盒 (ABIN8055816)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- Sandwich Human GSTM1 Kit to quantitate Human GSTM1 in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: M1-K218Capture antibody and Detection antibody: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Glutathione S-Transferase mu 1 (GSTM1) ELISA Kit
GSTM1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Glutathione S-Transferase mu 1 (GSTM1) ELISA KitGSTM1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glutathione S-Transferase mu 1 (GSTM1) ELISA KitGSTM1 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutathione S-Transferase mu 1 (GSTM1) ELISA KitGSTM1 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Glutathione S-Transferase mu 1 (GSTM1) ELISA KitGSTM1 适用: 人 Colorimetric Sandwich ELISA 2-500 ng/mL Cell Culture Supernatant, Plasma, Serum
Glutathione S-Transferase mu 1 (GSTM1) ELISA KitGSTM1 适用: 大鼠 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum
Glutathione S-Transferase mu 1 (GSTM1) ELISA KitGSTM1 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 5.1%, 4.5%, 5.9%
Inter-Assay Precision (CV%): 3.9%, 4.5%, 5.1% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))
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别名
- GSTM1
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背景
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Background: Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene.
Gene Full Name: glutathione S-transferase mu 1
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基因ID
- 2944
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UniProt
- P09488
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途径
- Negative Regulation of Transporter Activity
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