Factor XI ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for Factor XI ELISA 试剂盒 (ABIN8055801)
抗原
See all Factor XI (F11) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 0.78 ng/mL
-
原理
- Sandwich Human F11 ELISA Kit to quantitate Human F11 in cell culture supernatants, serum and plasma (heparin, EDTA).
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NS0
Immunogen sequence: E19-V625Capture antibody and Detection antibody: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
-
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 20 pg/mL
-
组件
-
- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
Coagulation Factor XI (F11) ELISA Kit
F11 适用: 小鼠 Colorimetric Competition ELISA 0.47 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor XI (F11) ELISA KitF11 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Coagulation Factor XI (F11) ELISA KitF11 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor XI (F11) ELISA KitF11 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor XI (F11) ELISA KitF11 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor XI (F11) ELISA KitF11 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma
Coagulation Factor XI (F11) ELISA KitF11 适用: 大鼠 Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Plasma
Coagulation Factor XI (F11) ELISA KitF11 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma
Coagulation Factor XI (F11) ELISA KitF11 适用: 猴 Colorimetric Sandwich ELISA 5.0-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Coagulation Factor XI (F11) ELISA KitF11 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
-
实验精密度
-
Intra-Assay Precision (CV%): 4.4%, 4.9%, 5.2%
Inter-Assay Precision (CV%): 5.5%, 4.6%, 5.7% -
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
-
有效期
- 12 months
-
-
- Factor XI (F11) (Coagulation Factor XI (F11))
-
别名
- F11
-
背景
-
Background: Factor XI or plasma thromboplastin antecedent is the zymogen form of factor XIa, one of the enzymes of the coagulation cascade. Like many other coagulation factors, it is a serine protease. In humans, Factor XI is encoded by the F11 gene. This gene encodes coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds. During activation of the plasma factor XI, an internal peptide bond is cleaved by factor XIIa (or XII) in each of the two chains, resulting in activated factor XIa, a serine protease composed of two heavy and two light chains held together by disulfide bonds. This activated plasma factor XI triggers the middle phase of the intrisic pathway of blood coagulation by activating factor IX. Defects in this factor lead to Rosenthal syndrome, a blood coagulation abnormality.
Gene Full Name: coagulation factor XI
-
基因ID
- 2160
-
UniProt
- P03951
抗原 See all Factor XI (F11) ELISA试剂盒
-
