AZGP1 ELISA 试剂盒
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Quick Overview for AZGP1 ELISA 试剂盒 (ABIN8055740)
抗原
See all AZGP1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human ZAG/AZGP1 ELISA Kit to quantitate Human AZGP1 in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: Q21-S298Capture antibody and Detection antibody: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 75 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1) ELISA Kit
AZGP1 适用: 人 Colorimetric Sandwich ELISA 4.7 ng/mL - 300 ng/mL Plasma, Saliva, Seminal Plasma, Serum, Sweat, Urine
alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1) ELISA KitAZGP1 适用: 小鼠 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum
alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1) ELISA KitAZGP1 适用: 人 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1) ELISA KitAZGP1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1) ELISA KitAZGP1 适用: 小鼠 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum
alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1) ELISA KitAZGP1 适用: 人 Colorimetric Sandwich ELISA 4.7 ng/mL - 300 ng/mL Plasma, Saliva, Seminal Plasma, Serum, Sweat, Urine
alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1) CLIA KitAZGP1 适用: 人 Chemiluminescent Sandwich ELISA 0.82 ng/mL - 600 ng/mL Plasma, Saliva, Seminal Plasma, Serum, Sweat, Urine
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 5.5%, 4.7%, 7.2%
Inter-Assay Precision (CV%): 7.4%, 5.6%, 8.0% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- AZGP1 (alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1))
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别名
- AZGP1
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背景
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Background: Zinc-alpha-2-glycoprotein is a protein that in humans is encoded by the AZGP1 gene. Alpha-2-glycoprotein, also known as AZGP1, belongs to the MHC class I family. It can be detected in body fluids such as serum, sweat, and seminal and breast cyst fluids. It has been shown that alpha-2-glycoprotein can stimulate lipolysis by adipocytes in vivo and in vitro. Thus it is believed that alpha-2-glycoprotein plays an important role in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and it also regulates melanin production by normal and malignant melanocytes. Alpha-2-glycoprotein is produced by both white and brown fat adipocytes and may act in a local autocrine fashion in the reduction of adiposity in cachexia.
Gene Full Name: alpha-2-glycoprotein 1, zinc-binding
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基因ID
- 563
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UniProt
- P25311
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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