Cadherin 4 ELISA 试剂盒
The 大鼠 Cadherin 4 ELISA Kit (ABIN8055700) is a Colorimetric ELISA Kit designed to quantify 大鼠 Cadherin 4.
产品编号 ABIN8055700
发货至:
中国
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中国
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Quick Overview for Cadherin 4 ELISA 试剂盒 (ABIN8055700)
抗原
See all Cadherin 4 (CDH4) ELISA试剂盒
Cadherin 4 (CDH4)
抗原表位
AA 167-731
适用
大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
156 pg/mL - 10000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Rat R-Cadherin-4 Cdh4 ELISA Kit to quantitate Rat Cdh4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: D167-A731Capture antibody and Detection antibody: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 50 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.2%, 4.3%, 4.1%
Inter-Assay Precision (CV%): 6.6%, 7.2%, 7.6% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- Cadherin 4 (CDH4)
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别名
- Cdh4
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背景
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Background: Cadherin-4 is a protein that in humans is encoded by the CDH4 gene. It is mapped to 3q43. This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. The encoded protein is involved in retinal angiogenesis during development where it plays a crucial role in the endothelial-astrocyte interactions. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene Full Name: cadherin 4
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基因ID
- 114588
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UniProt
- Q63149
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途径
- Cell-Cell Junction Organization, Regulation of Cell Size
抗原 See all Cadherin 4 (CDH4) ELISA试剂盒
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