SLIT2 ELISA 试剂盒
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Quick Overview for SLIT2 ELISA 试剂盒 (ABIN8055680)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Mouse SLIT2 ELISA Kit to quantitate Mouse Slit2 in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: Q26-Q900Capture antibody and Detection antibody: polyclonal antibody from sheep|a biotinylated polyclonal antibody from sheep
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 50 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Slit Homolog 2 (Drosophila) (SLIT2) ELISA Kit
SLIT2 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Slit Homolog 2 (Drosophila) (SLIT2) ELISA KitSLIT2 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Slit Homolog 2 (Drosophila) (SLIT2) ELISA KitSLIT2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Slit Homolog 2 (Drosophila) (SLIT2) ELISA KitSLIT2 适用: Pig Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Slit Homolog 2 (Drosophila) (SLIT2) ELISA KitSLIT2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Slit Homolog 2 (Drosophila) (SLIT2) ELISA KitSLIT2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Slit Homolog 2 (Drosophila) (SLIT2) ELISA KitSLIT2 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Slit Homolog 2 (Drosophila) (SLIT2) ELISA KitSLIT2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Slit Homolog 2 (Drosophila) (SLIT2) ELISA KitSLIT2 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Cell Culture Supernatant, Plasma, Serum
Slit Homolog 2 (Drosophila) (SLIT2) ELISA KitSLIT2 适用: 人 Colorimetric Sandwich ELISA 0.78-50 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.1%, 4.3%, 4.6%
Inter-Assay Precision (CV%): 5.8%, 6.9%, 7.2% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))
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别名
- Slit2
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背景
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Background: Slit homolog 2 protein is a protein that in humans is encoded by the SLIT2 gene. It is mapped to 5, 5 B3. The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants.
Gene Full Name: slit guidance ligand 2
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基因ID
- 20563
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UniProt
- Q9R1B9
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途径
- Regulation of Actin Filament Polymerization, Regulation of Cell Size, Smooth Muscle Cell Migration
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