TINAGL1 ELISA 试剂盒
The 人 TINAGL1 ELISA Kit (ABIN8055658) is a Colorimetric ELISA Kit designed to quantify 人 TINAGL1.
产品编号 ABIN8055658
发货至:
中国
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Our Local Distributor
中国
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北京 101111
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Quick Overview for TINAGL1 ELISA 试剂盒 (ABIN8055658)
抗原
See all TINAGL1 ELISA试剂盒
TINAGL1
(Tubulointerstitial Nephritis Antigen-Like 1 (TINAGL1))
抗原表位
AA 22-467
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
31.2 pg/mL - 2000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich Human TINAGL1 ELISA Kit to quantitate Human TINAGL1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: A22-H467Capture antibody and Detection antibody: a polyclonal antibody from sheep|a biotinylated polyclonal antibody from sheep
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.1%, 5.7%, 4.6%
Inter-Assay Precision (CV%): 5.8%, 6.9%, 6.4% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- TINAGL1 (Tubulointerstitial Nephritis Antigen-Like 1 (TINAGL1))
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别名
- TINAGL1
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背景
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Background: Tubulointerstitial nephritis antigen-like is a protein that in humans is encoded by the TINAGL1 gene. It is mapped to 1p35.2. The protein encoded by this gene is similar in sequence to tubulointerstitial nephritis antigen, a secreted glycoprotein that is recognized by antibodies in some types of immune-related tubulointerstitial nephritis. Three transcript variants encoding different isoforms have been found for this gene.
Gene Full Name: tubulointerstitial nephritis antigen like 1
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基因ID
- 64129
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UniProt
- Q9GZM7
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