LY9 ELISA 试剂盒
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Quick Overview for LY9 ELISA 试剂盒 (ABIN8055608)
抗原
See all LY9 (CD229) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 93.7 pg/mL
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原理
- Sandwich Mouse Ly9/SLAMF3/CD229 ELISA Kit to quantitate Mouse Ly9 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: K48-R453Capture antibody and Detection antibody: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 50 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Lymphocyte Antigen 9 (CD229) ELISA Kit
CD229 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Lymphocyte Antigen 9 (CD229) ELISA KitCD229 适用: 人 Colorimetric Sandwich ELISA 80-20.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Lymphocyte Antigen 9 (CD229) ELISA KitCD229 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.1%, 4.3%, 4.6%
Inter-Assay Precision (CV%): 5.8%, 6.9%, 6.4% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
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别名
- Ly9
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背景
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Background: T-lymphocyte surface antigen Ly-9 is a protein that in humans is encoded by the LY9 gene. This gene is mapped to 1 H3, 1 79.54 cM. Mature human CD229 consists of a 407 amino acid (aa) extracellular domain (ECD) with two Ig like V-set and two Ig like truncated C2-set domains. It also shows a 22 aa transmembrane segment, and a 179 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs. The ECD of human CD229 shares 57 % 59 % aa sequence identity with mouse and rat CD229. Within the first two Ig like domains that are common to all SLAM proteins, human CD229 shares 24 % 39 % aa sequence identity with human 2B4, BLAME, CD2F-10, CD84, CRACC, NTB-A, and SLAM. Alternate splicing generates two additional isoforms that lack the juxtamembrane Ig like domain or short cytoplasmic region. CD229 is expressed on T and B cells, thymocytes, and more weakly on NK cells. Homophilic binding between CD229 Molecules is mediated by the N-terminal Ig like domain. Human and mouse CD229 exhibit cross-species binding. Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell - B cell contact. Two tyrosines in the cytoplasmic domain are required for association of CD229 with the adaptor proteins AP-2 (μ2 chain) and GRB-2 and both are required for CD229 internalization.
Gene Full Name: lymphocyte antigen 9
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基因ID
- 17085
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UniProt
- Q01965
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途径
- BCR Signaling
抗原 See all LY9 (CD229) ELISA试剂盒
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