SCGB3A1 ELISA 试剂盒
大鼠 SCGB3A1 ELISA Kit, Colorimetric is an assay for quantification of 大鼠 SCGB3A1.
产品编号 ABIN8055566
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for SCGB3A1 ELISA 试剂盒 (ABIN8055566)
抗原
See all SCGB3A1 ELISA试剂盒
SCGB3A1
(Secretoglobin, Family 3A, Member 1 (SCGB3A1))
抗原表位
AA 22-104
适用
大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
15.6 pg/mL - 1000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
-
-
最低检测浓度
- 15.6 pg/mL
-
原理
- Sandwich Rat SCGB3A1 ELISA Kit to quantitate Rat SCGB3A1 in cell culture supernatants, serum and plasma (heparin or EDTA) .
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: E.coli
Immunogen sequence: F22-G104Capture antibody and Detection antibody: A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
-
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 10 pg/mL
-
组件
-
- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
-
实验精密度
-
Intra-Assay Precision (CV%): 4.7%, 5.2%, 5.7%
Inter-Assay Precision (CV%): 6.3%, 5.4%, 7.1% -
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
-
有效期
- 12 months
-
-
- SCGB3A1 (Secretoglobin, Family 3A, Member 1 (SCGB3A1))
-
别名
- SCGB3A1
-
背景
-
Background: Secretoglobin family 3A member 1 is a protein that in humans is encoded by the SCGB3A1 gene. It is mapped to 10q21. Members of the secretoglobin family, such as SCGB3A1, are secreted proteins of about 10 kD that are found in high concentrations in fluids of lung, lacrimal gland, salivary gland, prostate, uterus, and other tissues.
Gene Full Name: secretoglobin, family 3A, member 1
-
基因ID
- 338418
-
UniProt
- E9PU36
抗原 See all SCGB3A1 ELISA试剂盒
-
You are here:
