TREML2 ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 TREML2.
产品编号 ABIN8055549
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Quick Overview for TREML2 ELISA 试剂盒 (ABIN8055549)
抗原
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TREML2
(Triggering Receptor Expressed On Myeloid Cells-Like 2 (TREML2))
抗原表位
AA 19-268
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
62.5 pg/mL - 4000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich Human TREML2 ELISA Kit to quantitate Human TREML2 in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: G19-S268Capture antibody and Detection antibody: A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 50 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 6.7%, 5.2%, 5.7%
Inter-Assay Precision (CV%): 6.3%, 5.4%, 4.1% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- TREML2 (Triggering Receptor Expressed On Myeloid Cells-Like 2 (TREML2))
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别名
- TREML2
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背景
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Background: TREML2 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 (MIM 605085) and TREM2 (MIM 605086), but it has distinct structural and functional properties. This gene is mapped to 6p21.1.
Gene Full Name: triggering receptor expressed on myeloid cells like 2
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基因ID
- 79865
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UniProt
- Q5T2D2
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