PLA2G2A ELISA 试剂盒
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Quick Overview for PLA2G2A ELISA 试剂盒 (ABIN8055524)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich Mouse PLA2G2A ELISA Kit to quantitate Mouse Pla2g2a in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: N22-C146Capture antibody and Detection antibody: A polyclonal antibody from sheep|a biotinylated detection polyclonal antibody from sheep
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A) ELISA Kit
PLA2G2A 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A) ELISA KitPLA2G2A 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A) ELISA KitPLA2G2A 适用: 人 Colorimetric Sandwich ELISA 0.938 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A) ELISA KitPLA2G2A 适用: 大鼠 Colorimetric Sandwich ELISA 0.18 ng/mL - 12 ng/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A) ELISA KitPLA2G2A 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A) ELISA KitPLA2G2A 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A) ELISA KitPLA2G2A 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A) ELISA KitPLA2G2A 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A) ELISA KitPLA2G2A 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A) ELISA KitPLA2G2A 适用: 大鼠 Colorimetric Sandwich ELISA 0.18 ng/mL - 12 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.8%, 5.7%, 4.6%
Inter-Assay Precision (CV%): 5.1%, 5.9%, 6.4% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- PLA2G2A (Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A))
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别名
- Pla2g2a
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背景
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Background: Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene. It is mapped to 4 D3, 4 70.57 cM. Proteins belonging to the phospholipase A2 (PLA2) family hydrolyze phospholipids into sn2 fatty acids and lysophospholipids. They function in a variety of cellular processes, including the digestion of phospholipids and the production of molecules that induce inflammatory responses. This gene encodes a member of the group II class of secretory PLA2s. The secreted enzyme binds to heparin on the cell surface. Mutations in this gene increase the occurrence of intestinal polyps caused by a dominant mutation in the adenomatosis polyposis coli gene. A frameshift inactivates this gene product in some mouse strains including the strain of the reference genome, C57BL/6J, whereas a functional protein is produced in other strains.
Gene Full Name: phospholipase A2, group IIA (platelets, synovial fluid)
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基因ID
- 18780
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UniProt
- P31482
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途径
- Stem Cell Maintenance, Inositol Metabolic Process
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