PODXL2 ELISA 试剂盒
The 人 PODXL2 ELISA Kit (ABIN8055502) is a Colorimetric ELISA Kit designed to quantify 人 PODXL2.
产品编号 ABIN8055502
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for PODXL2 ELISA 试剂盒 (ABIN8055502)
抗原
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PODXL2
(Podocalyxin-Like 2 (PODXL2))
抗原表位
AA 33-500
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
156 pg/mL - 10000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human Endoglycan/PODXL2 ELISA Kit to quantitate Human PODXL2 in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: G33-T500Capture antibody and Detection antibody: A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 50 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.3%, 6.3%, 4.9%
Inter-Assay Precision (CV%): 4.4%, 6.3%, 7.4% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- PODXL2 (Podocalyxin-Like 2 (PODXL2))
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别名
- PODXL2
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背景
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Background: Podocalyxin like 2 is a protein that in humans is encoded by the PODXL2 gene. It is mapped to 3q21.3. This gene is a member of the CD34 family of cell surface transmembrane proteins, which are characterized by an N-terminal extracellular mucin domain, globular and stalk domains, a single pass transmembrane region, and a charged cytoplasmic tail. The encoded protein is a ligand for vascular selectins.
Gene Full Name: podocalyxin like 2
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基因ID
- 50512
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UniProt
- Q9NZ53
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