JAML ELISA 试剂盒
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Quick Overview for JAML ELISA 试剂盒 (ABIN8055450)
抗原
See all JAML (AMICA1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich Human AMICA/JAML ELISA Kit to quantitate Human JAML in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: L20-L275Capture antibody and Detection antibody: A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Adhesion Molecule, Interacts with CXADR Antigen 1 (AMICA1) ELISA Kit
AMICA1 适用: 人 Colorimetric Sandwich ELISA 0.41-100 ng/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.2%, 4.6%, 5.3%
Inter-Assay Precision (CV%): 6.4%, 6.8%, 7.2% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- JAML (AMICA1) (Adhesion Molecule, Interacts with CXADR Antigen 1 (AMICA1))
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别名
- JAML
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背景
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Background: JAML or Junctional Adhesion Molecule-Like, or AMICA1 is a JAM transmembrane protein family member. This gene is mapped to 11q23.3. It is composed of two extracellular immunoglobulin-like domains, a membrane-spanning region, and a cytoplasmic tail involved in activation signaling. A known ligand of JAML is Coxsackie virus and Adenovirus Receptor (CXADR in humans and CAR in mice) which has been shown to localize to the tight junctions of epithelial cells. JAML-mediated activation of CAR is required for neutrophil extravasation in addition to other leukocyte/epithelial cell interaction models. Other members of the JAM family of transmembrane proteins include JAM1, JAM2 and JAM3.
Gene Full Name: junction adhesion molecule like
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基因ID
- 120425
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UniProt
- Q86YT9
抗原 See all JAML (AMICA1) ELISA试剂盒
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