beta-2 Microglobulin ELISA 试剂盒
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Quick Overview for beta-2 Microglobulin ELISA 试剂盒 (ABIN8055409)
抗原
See all beta-2 Microglobulin (B2M) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human B2M / beta 2 Microglobulin ELISA Kit to quantitate Human B2M in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
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Analytical Method
- Quantitative
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特异性
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Target protein isolated from human plasma
Capture antibody and Detection antibody: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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beta-2-Microglobulin (B2M) ELISA Kit
B2M 适用: 人 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Plasma, Serum, Urine
beta-2-Microglobulin (B2M) ELISA KitB2M 适用: 人 Colorimetric Competition ELISA 0.025-10 μg/mL Serum, Urine
beta-2-Microglobulin (B2M) ELISA KitB2M 适用: 犬 Colorimetric Sandwich ELISA 0.78 μg/mL - 50 μg/mL Plasma, Serum, Tissue Homogenate
beta-2-Microglobulin (B2M) ELISA KitB2M 适用: 小鼠 Colorimetric Sandwich ELISA 0.41 ng/mL - 300 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
beta-2-Microglobulin (B2M) ELISA KitB2M 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
beta-2-Microglobulin (B2M) ELISA KitB2M 适用: 猴 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
beta-2-Microglobulin (B2M) ELISA KitB2M 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
beta-2-Microglobulin (B2M) ELISA KitB2M 适用: 小鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
beta-2-Microglobulin (B2M) ELISA KitB2M 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
beta-2-Microglobulin (B2M) ELISA KitB2M 适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 100 ng/mL Plasma, Serum, Urine
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.5%, 5.8%, 4.3%
Inter-Assay Precision (CV%): 8.7%, 6.3%, 6% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
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别名
- B2M
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背景
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Background: Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Gene Full Name: beta-2-microglobulin
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基因ID
- 567
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UniProt
- P61769
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途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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