TTR ELISA 试剂盒
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Quick Overview for TTR ELISA 试剂盒 (ABIN8055408)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- Sandwich Human Transthyretin / TTR / PreAlbumin ELISA Kit to quantitate Human TTR in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: G21-E147Capture antibody and Detection antibody: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Transthyretin (TTR) ELISA Kit
TTR 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Transthyretin (TTR) ELISA KitTTR 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transthyretin (TTR) ELISA KitTTR 适用: Cow Colorimetric Competition ELISA 312.5 ng/mL - 5000 ng/mL Plasma, Serum, Tissue Homogenate
Transthyretin (TTR) ELISA KitTTR 适用: 大鼠 Colorimetric Competition ELISA 375 ng/mL - 6000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Transthyretin (TTR) ELISA KitTTR 适用: 犬 Colorimetric Competition ELISA 0.625 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
Transthyretin (TTR) ELISA KitTTR 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Transthyretin (TTR) ELISA KitTTR 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Transthyretin (TTR) ELISA KitTTR 适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 100 ng/mL Plasma, Serum, Urine
Transthyretin (TTR) ELISA KitTTR 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 ng/mL - 500 ng/mL Plasma, Serum, Urine
Transthyretin (TTR) ELISA KitTTR 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 500 ng/mL Plasma, Serum, Urine
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.5%, 3.4%, 6.4%
Inter-Assay Precision (CV%): 6.3%, 2%, 3.3% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- TTR (Transthyretin (TTR))
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别名
- TTR
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背景
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Background: Transthyretin (TTR) is also known as prealbumin. It is mapped to chromosome region 18q11.2-q12.1. This gene encodes a carrier protein responsible for the transport of thyroid hormones and retinol. The protein consists of a tetramer of identical subunits. Due to increased stability of the tetramer form of this encoded protein in mouse, compared to the human protein, this gene product has a reduced tendency to form amyloid fibrils. In humans, this protein binds beta-amyloid preventing its aggregation and providing a neuroprotective role in Alzheimer's disease.
Gene Full Name: transthyretin
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基因ID
- 7276
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UniProt
- P02766
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途径
- Hormone Transport
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