Pyruvate Dehydrogenase (PDH) Activity Assay Kit
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北京 101111
Quick Overview for Pyruvate Dehydrogenase (PDH) Activity Assay Kit (ABIN7850088)
抗原
See all PDP 试剂盒检测方法
应用范围
样品类型
Analytical Method
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检测范围
- 0.28 U/L - 168.91 U/L
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最低检测浓度
- 0.28 U/L
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原理
- Pyruvate Dehydrogenase (PDH) Activity Assay Kit
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产品特性
- Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm.
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实验时间
- 15 min
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实验精密度
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Inter-assay precision: 10%
Intra-assay precision: 2%
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限制
- 仅限研究用
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注意事项
- Protect from light.
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储存条件
- -20 °C
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储存方法
- This product can be stored at -20°C for 12 months with shading light.
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有效期
- 12 months
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- PDP (Pyruvate Dehydrogenase Phosphatase (PDP))
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别名
- PDH
抗原
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