Phospholipase D (PLD) Activity Colorimetric Assay Kit
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北京 101111
Quick Overview for Phospholipase D (PLD) Activity Colorimetric Assay Kit (ABIN7850075)
抗原
See all Phospholipase D (PLD) 试剂盒检测方法
应用范围
样品类型
Analytical Method
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检测范围
- 0.68 U/L - 16.73 U/L
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最低检测浓度
- 0.68 U/L
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原理
- Phospholipase D (PLD) Activity Colorimetric Assay Kit
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产品特性
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Phospholipase D (PLD) exists widely in natural plants and animals, and PLD plays a key role in phospholipase metabolism. PLD hydrolyzes phosphodiester bonds of glycerol phospholipids to produce phosphatidylic acid and nitrogenous base. Abnormal PLD activity and expression are associated with Alzheimer's disease, stroke, cancer, and other brain diseases.
The detection principle of this kit is that PLD catalyze the substrate and the chromogenic agent to product colored substance, which has the maximum absorption at the wavelength of 550 nm. By measuring the OD value at 550 nm, PLD activity in the sample is calculated according to the standard curve.
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实验时间
- 1 h
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实验精密度
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Inter-assay precision: 1.8-9.5%
Intra-assay precision: 1.7-2.8%
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限制
- 仅限研究用
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注意事项
- Protect from light.
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储存条件
- -20 °C
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储存方法
- This product can be stored at -20°C for 12 months with shading light.
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有效期
- 12 months
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- Phospholipase D (PLD)
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别名
- Phospholipase D
抗原
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