NAD+/NADH Colorimetric Assay Kit (WST-8)
BCA
Colorimetric
Cell Samples, Tissue Samples
Quantitative
产品编号 ABIN7850058
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for NAD+/NADH Colorimetric Assay Kit (WST-8) (ABIN7850058)
抗原
NAD/NADH
检测方法
Colorimetric
应用范围
Biochemical Assay (BCA)
样品类型
Cell Samples, Tissue Samples
Analytical Method
Quantitative
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检测范围
- 0.02 μmol/L - 5.0 μmol/L
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最低检测浓度
- 0.02 μmol/L
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原理
- NAD+/NADH Colorimetric Assay Kit (WST-8)
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产品特性
- Detect total content of NAD+ and NADH Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NAD+ and NADH can be quantified by measure the OD value at 450 nm. Detect NADH After treating sample, heat at 60oC water bath for 30 min. the NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form yellow product, and the amount of NADH is determined by measure the OD value at 450 nm. Detect NAD+ and NAD+/NADH The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained of the first two steps as well as the separate content of NADPH. Note: NADP+ and NADPH have no effect on the determination results.
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NAD/NADH Assay Kit
BCA Cell Extracts
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实验时间
- 1 h
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实验精密度
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Inter-assay precision: 9.1%
Intra-assay precision: 1.8%
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限制
- 仅限研究用
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注意事项
- Protect from light.
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储存条件
- -20 °C
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储存方法
- This product can be stored at -20°C for 12 months with shading light.
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有效期
- 12 months
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- NAD/NADH
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别名
- NAD+/NADH
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物质类
- Chemical
抗原
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